Characterization of rumen microbiome and metabolome from oro …
Apr 11, 2023 · Less invasive rumen sampling methods, such as oro-esophageal tubing, became widely popular for exploring the rumen microbiome and metabolome. However, it remains …
Understanding Rumen Microbiology: An Overview - MDPI
Dec 18, 2023 · The rumen is the largest of the four chambers of the “stomach” in ruminant animals, which harbors an incredibly dense, diverse, and dynamic microbial community crucial …
Rumen sampling methods bias bacterial communities observed
May 5, 2022 · In this study, we determined how sampling method (oral stomach tubing vs cannulated grab sample), as well as rumen fraction type (liquid vs solid), bias the bacterial and …
two different rumen sample collection methods. Three ruminally cannulated lactating dairy cows were used as rumen fluid donors in a 3 × 3 Latin Square design. The treatments were rumen …
menocentesis (rumen puncture), an invasive technique that requires surgical centesis of the rumen and suffers the risk of localized abscesses or peritonitis (Duffield et al., 2004) and might …
Oro-ruminal sampling device and technique for rapid collection of rumen …
Nov 1, 2024 · We developed an intra-esophageal tube rumen sampling technique and device referred to as the “Rumen Sampler MG” with the aim of increasing the recovery of solid …
Hence, a less invasive alternative has been developed: the oral stomach tubing (OST) technique. The OST technique allows rumen fluid to be obtained through the oesophagus.
Comparison of Two Sampling Techniques for Evaluating Ruminal ...
Dec 22, 2020 · Rumen cannulation (RC) or stomach tubing (ST) are the two main techniques used to study ruminal fermentation and microbial community composition (Ramos-Morales et …
Low-cost sample preservation methods for high-throughput …
Jun 6, 2022 · For the GRC method, rumen samples were taken directly from the rumen via stomach tubing or at slaughter, and the freeze-dried sample was used for DNA extraction, …
Rumen sampling methods bias bacterial communities observed
May 5, 2022 · Sequencing of the 16S rRNA gene provides a powerful tool to survey the bacterial and some archaeal. Oral stomach tubing a cow to collect a rumen sample is a rapid, cost …