Making a Peripheral Blood Smear – Hematology Laboratory …
A small drop of EDTA-anticoagulated blood or capillary blood is placed on a clean microscope slide and spread across the slide creating a thin smear with a rounded “feathered edge’. Once the blood smear dries it can be stained using a common Wright stain (modified Romanowsky stain).
Technique and Tips for Making Perfect Blood Smears - Bitesize Bio
Jul 27, 2020 · Making blood smear slides can be critically important to hematologic investigation and diagnosis. They can be a useful tool to assess everything from white blood cell disorders, such as leukemias, to various types of acquired or congenital anemias, to broader organ issues like liver or kidney disease.
Blood Smear: Why It’s Done and What It Can Show - Verywell …
Feb 29, 2024 · A blood smear is a test that allows a healthcare provider to take a close look at a blood sample under a microscope. Up close, the smear shows how many of each type of blood cell are present. The sizes, shapes, and colors of the cells can be seen, along with any parasites or fragments in the blood.
CDC - DPDx - Diagnostic Procedures - Blood Specimens
Lay the slides flat and allow the smears to dry thoroughly (protect from dust and insects!). Insufficiently dried smears (and/or smears that are too thick) can detach from the slides during staining. The risk is increased in smears made with anticoagulated blood.
Blood Smear in Microscopy - Process/Technique, Artifacts/Refractiles
Proceeding with the 45 degree wedge or push slide technique used in manual and automated environments, creates a monolayer blood smear. This is done in a smooth and quick motion. Fixation, staining, washing and air drying are quickly commenced. Below is a very quick video on blood smear technique:
Blood Smear - Understand the Test - Testing.com
Sep 13, 2022 · A blood smear is used to evaluate your red blood cells (RBCs), noting any abnormal differences in size, shape, or other physical appearances such as that seen in various anemias, sickle cell disease, Thalassemia, or other disorders.
Preparation of Peripheral Blood Smears | My Hematology
The peripheral blood smear, a deceptively simple technique, allows for the visualization of the various blood cells found in whole blood. Its core principle lies in the creation of a thin, uniform film of blood cells spread upon a glass slide.
Blood Smear Preparation - Clinician's Brief
Clean, high-quality glass microscope slides are essential to blood smear preparation. Glass slides should be free of chipped edges, glass fragments, dust, powder, fingerprints, and other debris, as these can affect blood spreading.
Place a drop of blood approximately 4 mm in diameter on the slide (near the end if one smear is to be made, or at the proper location if two smears are to share a slide). See the drawing below.
Clean, high-quality glass microscope slides are essen-tial to blood smear preparation. Glass slides should be free of chipped edges, glass fragments, dust, powder, ingerprints, and other debris, as these can afect blood spreading.