Visualizing Mitochondrial Form and Function within the Cell
The ideal marker of mitochondrial energetic function would be a direct measure of how fast mitochondria are making ATP. Unfortunately, a robust, spatially-resolved measure of the rate …
Imaging Adenosine Triphosphate (ATP) - PMC - PubMed Central …
Quantitative imaging could be improved by engineering sensors with (1) a variety of ATP affinity ranges, (2) faster ATP binding and response kinetics, (3) higher brightness and contrast ratios, …
Visualization of ATP levels inside single living cells with ...
Adenosine 5′-triphosphate (ATP) is the major energy currency of cells and is involved in many cellular processes. However, there is no method for real-time monitoring of ATP levels inside …
Measurement of ATP concentrations in mitochondria of living …
Jan 1, 2020 · Another method to measure intracellular ATP levels is to image the ATP concentration in cells using a fluorescence microscope and genetically encoded fluorescent …
Real-Time Imaging of Mitochondrial ATP Dynamics Reveals the …
Oct 9, 2018 · This article uses organelle-targeted ATP biosensors to evaluate cellular metabolic settings with high resolution in real time. It shows that mitochondria supply ATP for glucose …
Mar 27, 2024 · The paper reports the first cryo-ET structure of the intact ATP synthase complex in mitochondria of the unicellular alga Polytomella. It reveals the rotary states of the central stalk, …
Mitochondrial morphology provides a mechanism for energy …
Dec 4, 2019 · From the matrix, ATP is transported into the intermembrane space (IMS) by the ATP/ADP translocator (ANT) in exchange to an ADP or ATP molecule. To reach the cytosol, …
Diversity in ATP concentrations in a single bacterial cell population ...
Oct 6, 2014 · In this study, we developed a new genetically-encoded ratiometric fluorescent ATP indicator “QUEEN”, which is composed of a single circularly-permuted fluorescent protein and …
Structure of the mitochondrial ATP synthase by electron …
Dec 1, 2003 · The mitochondrial adenosine triphosphate synthase (ATP synthase) is a membrane-bound multisubunit enzyme that couples the proton motive force across the inner …
Dynamic electron microscopy of ATP-induced myosin head movement ... - PNAS
Apr 29, 1997 · The time required for the released ATP to reach the filaments by diffusion was estimated to be less than 30 s by videorecording the ATP-induced myofibrillar shortening in …